Incontinencia urinaria en trabajadoras de la salud como factor predisponente de presentismo en Chile: estudio exploratorio de método mixto / Urinary incontinence in health workers as a predisposing factor for presenteeism in Chile: A mixed-methods exploratory study

Introducción La incontinencia urinaria impacta de forma negativa la calidad de vida de quienes la padecen y puede perjudicar las actividades laborales, siendo causante de presentismo en las profesionales de salud. Esto puede implicar la disminución en la calidad de la atención y seguridad de la/el paciente. El objetivo del presente estudio es explorar la autopercepción de las trabajadoras de salud que padecen incontinencia urinaria como factor predisponente de presentismo. Métodos Estudio mixto de carácter exploratorio-descriptivo. La muestra fue seleccionada de forma no probabilística e intencionada por criterio y conveniencia con un tamaño de 14 voluntarias, considerando la saturación de la información. Para el proceso y análisis de datos temáticos se consideraron los criterios de confiabilidad definidos por Guba. Resultados Muestra con edad media de 38,9 + 7,1 años y un puntaje de SPS-6 medio de 15,8 + 3,5 puntos, mostrando mayor alteración en la dimensión de evitar la desconcentración. Las narrativas presentes en el caso estudiado aportaron información relevante de cómo la incontinencia urinaria afecta el desempeño laboral de las trabajadoras de salud a través de la interrupción en su jornada, disminución en la calidad de la atención clínica, como también el aumento de su ansiedad respecto a su entorno. Conclusiones Dado que la incontinencia urinaria y el presentismo son experiencias subjetivas y multidimensionales, al igual que el efecto negativo en el desempeño laboral, se recomienda un estudio que permita identificar variables predictoras y las pérdidas económicas asociadas a esta condición. Con ello se buscaría establecer mejoras en el ambiente laboral, así como en el autocuidado de funcionarias, procurando mayores beneficios y mejores niveles de eficiencia en la organización.

Introduction Urinary incontinence negatively impacts the quality of life and can harm work activities, causing presenteeism in health professionals and decreasing the quality of care and patient safety. The objective of this study is to explore the self-perception of health workers who suffer from urinary incontinence as a predisposing factor for presenteeism. Methods Mixed study of an exploratory-descriptive nature. The sample was selected in a non-probabilistic and intentional way by criterion and convenience with a size of 14 volunteers, considering the saturation of the information. Reliability criteria defined by Guba for the process and analysis of thematic data were considered. Results The sample had a mean age of 38.9 + 7.1 years and a mean SPS-6 score of 15.8 + 3.5 points, showing alteration in the dimension of avoiding deconcentration. The narratives in the case study provide relevant information on how urinary incontinence affects the work performance of health workers through the interruption in their day, decreases the quality of clinical care, and increases their anxiety regarding their environment. Conclusions Urinary incontinence and presenteeism are subjective, and multidimensional experiences affect work performance. Therefore, further studies are recommended to identify predictor variables and the economic losses associated with this condition to establish improvements in the work environment and the self-care of female employees seeking greater benefits and better levels of efficiency in the organization

Assessment of Efficiency of Bioactive Glass, Self-Assembling Peptide, and Ozone Remineralising Agents on Artificial Carious Lesion

ABSTRACT Objective: To assess the efficacy of bioactive glass, self-assembling peptide, and ozone-remineralizing agents on the artificial carious lesion. Material and Methods: On the extracted 60 premolar teeth, an artificial carious lesion/demineralization was created. Later, the remineralization of demineralized teeth was done with respective remineralizing agents (Group A: Calcium sodium phosphosilicate (bioactive glass), Group B: Self-assembling peptide, Group C: Ozone remineralizing agents and Group D (Control): De ionized water. The degree of demineralization and remineralization were evaluated using the Vickers Hardness Number. Results: There was a decrease in microhardness from baseline to demineralization in all the groups, and this reduction was found to be statistically considerable. After the remineralization of demineralized samples with respective remineralizing agents, there was an increase in microhardness of 312.38, 276.67, and 254.42 in groups A, B, and C, respectively. In contrast, in Group D, there were no changes. Conclusion: Bioactive glass and self-assembling peptides had higher remineralizing capacities, which can be used to treat early carious lesions.

Research progress on dental materials for preventing root caries / 口腔疾病防治

@#The high incidence and untreated rate of root caries, a common and frequently occurring oral disease with challenging treatment in elderly individuals, is the main cause of tooth loss among elderly people, as rapid development results in pulpitis and periapical periodontitis or residual crown and root, which has been regarded as one of the common chronic oral diseases seriously affecting the quality of life of elderly people. Thus, early intervention and prevention are important. Traditional dental materials for preventing root caries have been widely used in clinical practice; however, they have the disadvantages of tooth coloring, remineralization and low sterilization efficiency. A series of new dental materials for preventing root caries have gradually become a research hotspot recently, which have the advantages of promoting the mineralization of deep dental tissue, prolonging the action time and enhancing adhesion. Future caries prevention materials should be designed according to the characteristics of root surface caries and the application population and should be developed toward simplicity, high efficiency and low toxicity. This review describes current research regarding anti-caries prevention material application, serving as a theoretical underpinning for the research of root caries prevention materials, which is important for both promotion in the effective prevention of root caries and improvement in the status of oral health and the quality of life among old people.

Enfoque hacia el uso de energías alternativas en establecimientos de atención médica / Approach to the use of alternative energies in medical care facilities

Introducción: El tema del cambio climático y sus efectos, en la salud, educación y transporte, es un tema emergente, que pretende la optimización del consumo y la eficiencia energética. Esta investigación se plantea como objetivo,la caracterización del uso y aprovechamiento de energías, en establecimientos de atención médica de la región capital durante el año 2022, considerando la distribución energética, eficiencia y fuentes primarias de energía utilizadas en este país. Métodos: Se trata de una investigación descriptiva, transversal y prospectiva,a través del análisis cuali-cuantitativo, con el uso de informantes clave quienes consideran importante la iluminación natural en los espacios y el mayor aprovechamiento energético en áreas como la quirúrgica y consulta externa. Resultados: Surge el uso de la energía solar, eólica e hidráulica como recursos energéticos aprovechables, así como la sostenibilidad y la mantenibilidad en el diseño y rediseño de infraestructuras hospitalarias. Los tipos de energías utilizados en Venezuela, siguen correspondiendo ala hidráulica y combustibles fósiles, se conoce la tecnología e implementación de paneles solares para la mejoría del cambio climático, la huella del carbono, el uso de energías verdes y reducción de combustibles fósiles. Su aceptación depende de regulaciones y la concientización energética como elementos fundamentales para el cambio.

Introduction: The issue of climate change and itseffects, in health, education and transportation, is an emergingissue, which aims at the optimization of energy consumption andefficiency. e objective of this research is to characterize the useand exploitation of energy in health care facilities in the capitalregion during the year 2022, considering the energy distribution,efficiency and primary energy sources used in this country.Methods: This is a descriptive, cross-sectional and prospectiveresearch, through qualitative-quantitative analysis, with the useof key informants who consider important the natural lightingin the spaces and the greater use of energy in areas such assurgery and outpatient care. Results: The use of solar, windand hydraulic energy emerged as usable energy resources, aswell as sustainability and maintainability in the design andredesign of hospital infrastructures. The types of energy used inVenezuela continue to correspond to hydraulics and fossil fuels; the technology and implementation of solar panels is known forthe improvement of climate change, the carbon footprint, theuse of green energy and reduction of fossil fuels. Their acceptancedepends on regulations and energy awareness as fundamental elements for change.

Metrics of efficient use of operating rooms during the SARS-CoV-2 (COVID-19) pandemic / Métrica de indicadores de uso eficiente de quirófano durante la pandemia por SARS-CoV-2 (COVID-19)

Abstract: Introduction: the COVID-19 pandemic has induced a transformation in the way hospitals function, causing a decrease in the time and efforts dedicated to surgical activity, which in turn has caused delays in the surgery schedule of most hospitals. This represents a major public health problem, significantly compromising the principle of equity that inspires public health systems throughout the world. To address this problem, it would be of the utmost importance to put in place initiatives to measure and improve surgical efficiency. Objective: evaluate indicators of efficiency in the use of operating rooms during the COVID-19 pandemic. Material and methods: a descriptive, longitudinal retrospective study was conducted on 3554 patients scheduled for surgery during a one-year period of the COVID-19 pandemic. Indicators of efficiency in they use of operating rooms were measured. The data was processed using SPSS v-25.0. Results: a total of 3,554 surgeries were scheduled, 1,309 of them emergency surgeries, 1,979 elective surgeries, and 266 deferred surgeries. The following parameters were estimated: Starting time of the procedure (42.32 ± 37.04 min); opportunity for emergency surgeries (104.69 ± 102.55 min); starting time of anesthesia (10.11 ± 9.85 min); starting time of surgery (40.03 ± 24.68 min); time of admission to post-anesthesia care unit/intensive care unit (PACU/ICU) (15.35 ± 29.94 min); turnover or replacement time (177.97 ± 174.33 min); active surgery time (27.70%). Conclusions: the COVID-19 pandemic negatively impacted the indicators of efficient use of operating rooms, posing new challenges for the management and organization of surgical work.

Resumen: Introducción: la pandemia por COVID-19 ha emplazado una transformación hospitalaria, esto acarreó un decremento de la actividad quirúrgica e implicó un aplazamiento en la programación, lo que representó un problema, ya que comprometió sensiblemente el principio de equidad que inspira a los sistemas sanitarios. Así, resultó imperativa la implementación de iniciativas para medir y mejorar la eficiencia quirúrgica. Objetivo: medir los indicadores de uso eficiente del quirófano durante la pandemia por COVID-19. Material y métodos: se realizó un análisis descriptivo, longitudinal y retrospectivo en 3,554 pacientes programados para cirugía, durante la pandemia en un período de un año, además se midieron los indicadores de uso eficiente del quirófano. Los datos fueron procesados en SPSS v-25.0. Resultados: se programaron 3,554 cirugías, 1,309 urgencias, 1,979 electivas, 266 diferidas. Se estimó un tiempo de inicio del procedimiento 42.32 ± 37.04 min, oportunidad para urgencias quirúrgicas 104.69 ± 102.55 min, tiempo de inicio de anestesia 10.11 ± 9.85 min, tiempo de inicio de cirugía 40.03 ± 24.68 min, tiempo para la admisión en la unidad de cuidados postanestésicos/unidad de terapia intensiva (UCPA/UTI) 15.35 ± 29.94 min, tiempo de rotación o recambio 177.97 ± 174.33 min y tiempo quirúrgico activo 27.70%. Conclusiones: la pandemia por COVID-19 impactó negativamente en los indicadores de uso eficiente del quirófano, lo que implicará nuevos retos en la gestión y organización de la jornada quirúrgica para su mejora.

Eficiência em saúde pública: a trajetória de um conceito proveniente da engenharia / Efficiency in public health: the trajectory of a concept from engineering

RESUMO A temática mais geral desta pesquisa diz respeito à eficiência na administração pública e sua manifestação na saúde pública. Em um contexto neoliberal, marcado por políticas de austeridade, em que as restrições orçamentárias em relação às políticas sociais são um dos aspectos principais, a análise sobre o que significa eficiência torna-se uma questão de pesquisa relevante. As diferentes ideias sobre eficiência, constituídas e modificadas ao longo da história, exercem influência na gestão dos recursos públicos. Neste sentido, este artigo tem como objetivo descrever a trajetória de desenvolvimento do conceito de eficiência na administração pública, mais especificamente na área de saúde pública. Para alcançar este objetivo, foi realizado um ensaio teórico, no qual desenvolve-se um argumento teórico-analítico destinado a orientar pesquisas empíricas em relação ao tema da eficiência na gestão em saúde. Este argumento é baseado em três pressupostos: ao longo de um século de desenvolvimento do conceito de eficiência na administração pública, este conceito ainda é fortemente carregado de pressupostos oriundos da engenharia; esses pressupostos, por sua vez, se conectam diretamente com princípios da economia neoclássica, que estão na base de perspectivas neoliberais aplicadas à gestão pública; e na área da saúde, o conceito de eficiência fundado apenas em pressupostos da economia de mercado é insuficiente, necessitando ser articulado aos conceitos de eficácia e efetividade.

ABSTRACT This article aims to describe the development trajectory of the concept of efficiency in public administration, more specifically in the area of public health. To achieve this objective, a theoretical essay was carried out, in which a theoretical-analytical argument was developed to guide empirical research in relation to the issue of efficiency in health management. This argument is based on three assumptions: over a century of development of the concept of efficiency in public administration, this concept is still heavily loaded with assumptions from engineering; these assumptions, in turn, are directly connected with principles of neoclassical economics, which underlie neoliberal perspectives applied to public management; and in the health area, the concept of efficiency based only on market economy assumptions is insufficient, needing to be articulated with the concepts of efficacy and effectiveness.

Satisfacción del usuario en la atención odontológica comunitaria en Reynosa, Tamaulipas / User ́s satisfaction of community dental care in Reynosa,Tamaulipas

Resumen Objetivo: Evaluar el nivel de satisfacción en la atención recibida por los usuarios de la Clínica Odontológica de la Universidad México Americana del Norte (CO-UMAN) Allende. Material y método: El estudio fue descriptivo, observacional, transversal y prospectivo; se aplicó una encuesta de opinión a 200 pacientes, seleccionados mediante muestreo por conveniencia en 2019, sobre la estructura, proceso y resultados de la atención; así como bio-demográficos de cada paciente. Resultados: Predominaron pacientes con edades entre 21 y 60 años (71%), mujeres (63%), casados(as) (45%), empleados(as)/amas de casa (57%) y baja frecuencia de enfermedades concomitantes (10%). A mayor edad hubo mayor frecuencia de enfermedades concomitantes (r=0.26; p<0.05) y satisfacción en el servicio (r=0.26; p<0.05); con otras enfermedades concomitantes la satisfacción del paciente fue menor (r=-0.39; p<0.05). Los pacientes indican que hay más satisfacción (de 2.6 a 5.4 veces) al recibir un servicio adecuado o mejor. La satisfacción del paciente y la calidad de la atención de la clínica se asoció positivamente (p<0.05) con los trámites sencillos, trato por parte del odontólogo; confianza en la calidad de los servicios recibidos; relación costo/beneficio; puntualidad en la atención y efectividad del tratamiento odontológico recibido. Los coeficientes canónicos estandarizados indicaron mayor contribución en la satisfacción del usuario: las instalaciones e infraestructura de la clínica (0.479); atención del personal y proceso administrativo (0.543); atención del odontólogo (0.700); confianza en la atención y percepción del servicio recibido (0.660) y pobre relación con edad (0.078), género (0.030), estado civil (-0.040) y ocupación del paciente (0.065). Conclusión: No obstante que la cantidad y calidad del servicio de la CO-UMAN se ha incrementado y mejorado sustantivamente, es necesario desarrollar un plan de mejora continua para alcanzar estándares de calidad total.

Abstract Objective: To evaluate the level of satisfaction in the care received by the users of the Dental Clinic of the Universidad México Americana del Norte (DC-UMAN) Allende. Material and method: The study was descriptive, observational, cross-sectional, and prospective. In 2019, an opinion survey on the structure, proceedings, and results of the care, as well as bio-demographics of each patient was applied to 200 patients selected by convenience sampling. Results: Patients aged between 21 and 60 years (71%), women (63%), married (45%), employees/housewives (57%) and a low frequency of concomitant diseases (10%) predominated. At an older age, there was a higher frequency of concomitant diseases (r=0.26; p<0.05) and satisfaction in the service (r=0.26; p<0.05); with other concomitant diseases, patient satisfaction was lower (r=-0.39; p<0.05). Patients indicated that there is more satisfaction (from 2.6 to 5.4 times) when receiving an adequate or better service. Patient satisfaction and the quality of clinic care were positively associated (p<0.05) with simple procedures, treatment by the dentist, confidence in the quality of the services received, cost-benefit ratio, punctuality in the care and effectiveness of the dental treatment received. The standardized canonical coefficients indicated a greater contribution to user satisfaction from the facilities and infrastructure of the clinic (0.479), staff attention and administrative process (0.543), dental care (0.700), trust in care and perception of the service received (0.660) and poor relationship with age (0.078), gender (0.030), marital status (-0.040), and occupation (0.065) of the patient. Conclusion: Although the quantity and quality of the DC-UMAN service has increased and improved substantially, it is necessary to develop a continuous improvement plan to achieve total quality standards.

Calidad nutricional e impacto en medio ambiente por los insumos de un comedor universitario en Uruguay / Nutritional quality and environmental impact by the inputs of a university canteen in Uruguay

El espacio de comedores son una forma de garantizar el acceso a alimentos seguros, saludables y medio ambientalmente responsables para los estudiantes universitarios. Objetivo. Este estudio tuvo como objetivo caracterizar los estándares del menú, a partir de la evaluación de la calidad nutricional y del impacto en el medio ambiente por los insumos utilizados para su elaboración por un Comedor Universitario (CU) en Uruguay. Materiales y métodos. Se trató de un estudio de caso retrospectivo, relativo al año 2021. Las variables evaluadas fueron a) cantidad total de alimento comprado en kilogramos (kg); b) cantidad en kilocalorías (kcal) e impactos ambientales por: c) generación de residuos; d) huella hídrica (HH); y e) gases de efecto invernadero (GEI). También se realizaron cálculos para evaluar la ecoeficiencia (EE). Resultados. El CU sirvió 33.740 comidas en 2021, de las cuales el 87,1% fueron almuerzos. De los 78 tipos de alimentos comprados, 41 de ellos representaron el 93,17% del total en kilogramos. En la calidad nutricional, menos del 10% se clasificaron como alimentos procesados o ultraprocesados. En la evaluación de aspectos nutricionales e impactos ambientales, los alimentos de origen animal representaron el 26,52% del total en kg y el 69,78% de los HH. Un alimento de origen vegetal tuvo el mayor impacto de GEI debido a su origen. En cuanto a la EE, la valoración general mostró un resultado de 0,0626 para los alimentos de origen animal y 0,3838 para los de origen vegetal. Conclusiones. Corresponde a los servicios de alimentación para colectividades considerar, en la planificación del menú, además de la calidad nutricional y sanitaria de la oferta alimentaria, los impactos ambientales que estas genera(AU)

The canteens are a way to guarantee access to safe, healthy and environmentally responsible food for university students. Objective. The purpose of this research was to characterize the menu standards, based on the evaluation of the nutritional quality and the environmental impact of the inputs used for its preparation by a university canteen in Uruguay. Materials and methods. This was a retrospective case study, relating to the year 2021. The variables evaluated were a) total amount of food purchased in kilograms (kg); b) quantity in kilocalories (kcal) and environmental impacts due to: c) waste generation; d) water footprint (WF); and e) greenhouse gases (GHG). Calculations were also performed to assess eco-efficiency (EE). Results. The university canteen served 33,740 meals in 2021, of which 87.1% were lunches. Of the 78 types of food purchased, 41 of them represented 93.17% of the total in kilograms. In nutritional quality, less than 10% are classified as processed or ultra-processed foods. In the evaluation of nutritional aspects and environmental impacts, foods of animal origin represented 26.52% of the total in kg and 69.78% of WF. A vegetal based food had the highest GHG impact due to its origin. Regarding the EE calculations, the general assessment showed a result of 0.626 for foods of animal origin and 0.3838 for those of vegetable origin. Conclusions. It corresponds to the food services for communities to consider, in the planning of the menu, in addition to the nutritional and sanitary quality of the food offer, the environmental impacts that it generates(AU)

Estimación de la eficiencia del uso de pabellones electivos en el sistema de salud público chileno entre 2018 y 2021 / Efficiency estimation on the use of elective surgical theaters in the Chilean public health system from 2018 to 2021

Objective The efficient use of wards intended for elective surgeries is essential to resolve cases on the surgical waiting list. This study aims to estimate the efficiency of ward use in the Chilean public health system between 2018 and 2021. Methods The design was an ecological study. Section A.21 of the database constructed by the monthly statistical summaries that each public health network facility reported to the Ministry of Health between 2018 and 2021 was analyzed. Data from subsections A, E and F were extracted: ward staffing, total elective surgeries by specialty, number and causes of suspension of elective surgeries. Then, the surgical performance during working hours and the percentage of hourly occupancy for a working day was estimated. Additionally, an analysis was made by region with data from 2021. Results The percentage of elective wards in use ranged from 81.1% to 94.1%, while those enabled for those staffing ranged from 70.5% to 90.4% during 2018 and 2021. The total number of surgeries was highest in 2019 (n = 416 339), but for 2018, 2020, and 2021 it ranged from 259 000 to 297 000. Suspensions varied between 10.8% (2019) and 6.9% (2021), with the leading cause being patient-related. When analyzing the number of cases canceled monthly by facility, we saw that the leading cause was trade union-related. The maximum throughput of a ward intended for elective surgery was reached in 2019 and was 2.5 surgeries; in 2018, 2020 and 2021, the throughput borders on two surgeries per ward enabled for elective surgery. The percentage of ward time occupied during working hours by contract day varies between 80.7% (2018) and 56.8% (2020). Conclusions All the parameters found and estimated in this study show that there is an inefficient utilization of operating rooms in Chilean public healthcare facilities.

Objetivo El uso eficiente de pabellones destinados a cirugías electivas es fundamental para resolver patologías en lista de espera quirúrgica. El objetivo general de este estudio es estimar la eficiencia del uso de pabellones en el sistema de salud público de Chile entre los años 2018 y 2021. Métodos El diseño fue un estudio ecológico. Se analizó la Sección A.21 de la base de datos construida por los resúmenes estadísticos mensuales que cada establecimiento de la red de salud pública reportó al Ministerio de Salud de Chile entre 2018 y 2021. Se extrajeron los datos de la subsección A, E y F: dotación de pabellones, total de cirugías electivas por especialidad, número y causas de suspensión de cirugías electivas. Luego se estimó el rendimiento quirúrgico en horario hábil y el porcentaje de ocupación horaria respecto de una jornada laboral. Adicionalmente, se hizo un análisis por región con datos de 2021. Resultados El porcentaje de pabellones electivos respecto de los en dotación varió entre 81,1 y 94,1%; mientras que los habilitados respecto de los en dotación varió entre 70,5 y 90,4% durante 2018 y 2021. El número total de cirugías fue más alto en 2019 (n = 416 339), pero en 2018, 2020 y 2021 variaron entre 259 y 297 mil cirugías. Las suspensiones varían entre 10,8 (2019) y 6,9%w(2021), siendo la principal causa de suspensión atribuida al "paciente". Al analizar la cantidad de pacientes suspendidos mensualmente por institución, se observa que la principal causa es "gremial". El rendimiento máximo de un pabellón destinado a cirugía electiva se alcanzó en 2019 y fue de 2,5 cirugías; mientras que en 2018, 2020 y 2021 el rendimiento bordea las dos cirugías por pabellón habilitado para cirugía electiva. El porcentaje de tiempo de pabellón ocupado en horario hábil respecto a una jornada de contrato varía entre 80,7 (2018) y 56,8% (2020). Conclusiones Todos los parámetros encontrados y estimados en este estudio muestran que el uso de pabellones en el sistema público de Chile es ineficiente.

Evaluación de la eficiencia de dos estructuras para jardines de coral de la especie Acropora cervicornis (Scleractinia: Acroporidae) en el Parque Nacional Portobelo

Introducción: En Panamá y el mundo, desde hace varios años, los arrecifes se han visto amenazados por acciones como el desarrollo costero, sedimentación, contaminación por entidades terrestres, que son las más frecuentes, dando lugar a la implementación de metodologías de restauración. Objetivo: Comparar la eficiencia de dos estructuras de jardines coralinos en el crecimiento de la especie Acropora cervicornis en Bahía Huertas, Parque Nacional Portobelo, Colón Panamá. Métodos: Se colocaron dos estructuras de tipo fijo, utilizando PVC junto con bases de concreto hidráulico, y dos estructuras tipo tendedero, utilizando PVC e hilo de nylon. Los fragmentos de coral que se colocaron en las estructuras provenían de Bahía Huertas e Isla Buenaventura. El crecimiento de los corales se determinó mediante el método de medición de crecimiento lineal y extensión tisular. Resultados: Se plantaron un total de 80 fragmentos de coral, lo que representa una extensión estimada de 9.71 m de coral en Bahía Huertas. Tras nueve meses de estudio, se estimó un total de 15.3 m de coral totalmente nuevo (1 525.6 cm), donde sólo el 4.6 % pertenecía a los fragmentos de la estructura de tipo fijo, mientras que el 95.4 % pertenecía a la estructura de tipo tendedero. Conclusiones: La estructura tipo tendedero fue la más eficiente y viable dentro del estudio, con mejor tasa de supervivencia y crecimiento en comparación con la estructura tipo fija, donde sí presentó una mortalidad del 45 %.

Introduction: In Panama and the world, for several years, reefs have been threatened by actions such as coastal development, sedimentation, and contamination by terrestrial entities, which are the most frequent, giving rise to the implementation of restoration methodologies. Objective: To compare the efficiency of two coral garden structures in the growth of the species Acropora cervicornis in Huertas Bay, Portobelo National Park, Colon Panama. Methods: Two fixed-type structures were placed, using PVC together with hydraulic concrete bases, and two clothesline-type structures, using PVC and nylon thread. The coral fragments that were placed in the structures came from Bahía Huertas and Buenaventura Island. The growth of the corals was determined using the linear growth and tissue extension measurement method. Results: A total of 80 coral fragments were planted, representing an estimated extension of 9.71 m of coral in Huertas Bay. After nine months of study, a total of 15.3 m of totally new coral (1 525.6 cm) was estimated, where only 4.6 % belonged to the fragments of the fixe-type structure, while 95.4 % belonged to the clothesline-type structure. Conclusions: The clothesline-type structure was the most efficient and viable within the study, with better survival and growth rate compared to the fixed type structure, where it did present a mortality of 45 %.

Challenges in estimating the encapsulation efficiency of proteins in polymersomes - Which is the best method?

Abstract Polymersomes are nanometric vesicles that can encapsulate large and hydrophilic biomolecules, such as proteins, in the aqueous core. Data in literature show large variation in encapsulation efficiency (%EE) values depending on the method used for calculation. We investigated different approaches (direct and indirect) to quantify the %EE of different proteins (catalase, bovine serum albumin-BSA, L-asparaginase and lysozyme) in Pluronic L-121 polymersomes. Direct methods allow quantification of the actual payload of the polymersomes and indirect methods are based on the quantification of the remaining non-encapsulated protein. The protein-loaded polymersomes produced presented approximately 152 nm of diameter (PDI ~ 0.4). Higher %EE values were obtained with the indirect method (up to 25%), attributed to partial entanglement of free protein in the polymersomes poly(Ethylene Glycol) corona. For the direct methods, vesicles were disrupted with chloroform or proteins precipitated with solvents. Reasonable agreement was found between the two protocols, with values up to 8%, 6%, 17.6% and 0.9% for catalase, BSA, L-asparaginase and lysozyme, respectively. We believe direct determination is the best alternative to quantify the %EE and the combination of both protocols would make results more reliable. Finally, no clear correlation was observed between protein size and encapsulation efficiency.

Estudo sobre o uso do Picture Archiving and Communication System (PACS) em um Hospital Universitário da cidade de Belo Horizonte, Minas Gerais, Brasil / A study on the use of the Picture Archiving and Communication System (PACS) at the Hospital the Federal University of Belo Horizonte city of Minas Gerais (HC-UFMG)

Estudo sobre o uso do Picture Archiving and Communication System (PACS) em um Hospital Universitário da Cidade de Belo Horizonte, Minas Gerais- Brasil. 2023. Trabalho de Conclusão de Curso (Mestrado Profissional em Trabalho e Gestão Participativa na Saúde) ­ Escola de Enfermagem da Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil. No contexto hospitalar, os exames de imagem são fundamentais para subsidiar e qualificar a tomada de decisão clínica. A radiologia, enquanto especialidade da medicina, tem como objetivo auxiliar os profissionais solicitantes na tomada de decisão para o diagnóstico. A radiologia é essencial porque, em diversas situações, é dela que deriva o tratamento. No contexto do Hospital das Clínicas da Universidade Federal de Minas Gerais (HC-UFMG), utiliza-se o sistema de arquivamento, comunicação de imagens médicas conhecido como- Picture Archiving and Communication System (PACS). Ele é usado no setor da radiologia, permitindo aos outros profissionais solicitantes o acesso e a análise das imagens médicas, de forma eficiente e segura. A pergunta que norteou este estudo foi: Quais as potencialidades e os desafios decorrentes do uso do PACS no setor de Radiologia do Hospital de Clínicas da Universidade Federal de Minas Gerais (HC-UFMG)? O objetivo da pesquisa foi analisar como foi a implantação e a implementação do uso do PACS no setor de radiologia do HC-UFMG. Trata-se de um estudo de natureza qualitativa, exploratória e descritiva. O cenário da pesquisa foi o setor de Radiologia do HC-UFMG. Os participantes foram nove médicos radiologistas do referido setor. Foi adotado como critério de inclusão: médicos radiologistas efetivos (médicos concursados e que não estavam em período de férias e ou afastados) que utilizavam o sistema PACS no setor da Radiologia do HC-UFMG. Como critérios de exclusão adotou-se: médicos que não compunham o quadro efetivo no setor, ou seja, que eram voluntários ou residentes; foram excluídos também dois médicos que estavam de férias e dois que estavam de licença formal durante o período da coleta de dados. A coleta de dados foi realizada por meio de entrevistas com roteiro semiestruturado e levantamento documental. Realizou-se a análise dos dados das entrevistas pela técnica de Análise de Conteúdo e, dos documentos, por análise documental. A pesquisa foi submetida ao Comitê de Ética em Pesquisa da Universidade Federal de Minas Gerais (COEP/UFMG) e aprovada pelo CAAE 67471123.0.0000.5149, após aprovação pelo Departamento de Gestão em Saúde e anuência da Gerência de Ensino e Pesquisa (GEP) do HC/UFMG. Os principais resultados indicam que o PACS é considerado uma ferramenta central e eficiente pelos radiologistas entrevistados, por facilitar o acesso e a visualização dos exames de imagem, agilizar a tomada de decisão clínica e contribuir para uma assistência mais coordenada e eficaz. Conclui-se que a relevância deste estudo se reflete na contribuição para a compreensão das potencialidades e desafios relacionados ao uso do PACS em um hospital universitário, fornecendo subsídios para aprimorar a gestão em saúde e a assistência aos pacientes. O produto técnico deste estudo é um relatório técnico, a ser apresentado à direção do HC/UFMG. Palavras-chave: Picture Archiving and Communication System (PACS). Tecnologia. Eficiência. Gestão em Saúde.

A study on the use of the Picture Archiving and Communication System (PACS) at the Hospital the Federal University of Belo Horizonte city of Minas Gerais (HC-UFMG). 2023. Dissertation (Professional Master's in Work and Participatory Management in Health) - School of Nursing, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. In the hospital context, imaging tests are essential to support and qualify clinical decision- making. Radiology, as a specialty of medicine, aims to assist referring physicians in making diagnostic decisions. Radiology is essential because in several situations, treatment is derived from it. In the context of Hospital das Clínicas of the Federal University of Minas Gerais (HC- UFMG), we used the Picture Archiving and Communication System (PACS) for archiving and communication medical images. Which is used in the radiology sector and allows other requesting professionals to access and analyze medical images efficiently and securely. A question that guided this study was: What are the potentialities and challenges resulting from the use of the future Archiving and Communication System (PACS) in the Radiology sector Clinical Hospital of Federal University of Minas Gerais (HC-UFMG)? The purpose of the research was to analyze the potentialities and challenges resulting from the use of PACS in the radiology at HC-UFMG. This is qualitative, exploratory and descriptive. The research scenario was the Radiology sector of HC-UFMG. The participants were nine radiologists from that sector. The following inclusion criteria were adopted: effective radiologists (doctors who were on public service and who were not on vacation and/or away) who used the PACS system in the Radiology sector of HC-UFMG. As exclusion criteria it was adopted: physicians who did not make up the effective physicians in the sector, that is, who were volunteers or residents; were excluded also two doctors who were on vacation and two who were on formal leave during the period of data collection. Data collection was carried out through interviews with a script semi-structured and documentary survey. Data analysis of the interviews was carried out by the Content Analysis technique and documents by document analysis. The research was submitted to the Research Ethics Committee of the Federal University of Minas Gerais (COEP/UFMG) and approved by CAAE 67471123.0.0000.5149, after approval by Department of Health Management and approval of the Teaching and Research Management (GEP) of the HC-UFMG The main results indicate that the PACS is considered a tool central and efficient by the radiologist interviewed, as it facilitates access and visualization of imaging exams, streamline clinical decision-making and contribute to more coordinated and effective. It is concluded that the relevance of this study is reflected in the contribution to the understanding of the potentialities and challenges related to the use of PACS in a hospital university, providing subsidies to improve health management and assistance to patients. The technical product of this study is a technical report, to be presented to the management from HC-UFMG. Keywords: Picture Archiving and Communication System (PACS). Technology. Efficiency. Health management.

Common mental disorders, productivity and presenteeism in nursing workers / Trastornos mentales comunes, productividad y presentismo en trabajadores de enfermería / Transtornos mentais comuns, produtividade e presenteísmo em trabalhadores de enfermagem

ABSTRACT Objective: To investigate the association between the occurrence of common mental disorders with loss of productivity and presenteeism in nursing workers at a public health service. Method: This is a cross-sectional study, with 291 workers from Midwestern Brazil. Data collection carried out from October 2019 to January 2020, with instruments for sociodemographic characterization, labor and health conditions: Self Reporting Questionnaire-20, Standford Presenteeism Scale and Work Limitations Questionnaire. Data were analyzed using descriptive and inferential statistics, with Mann-Whitney tests and logistic regression, respecting a significance level of 5%. Results: The occurrence of common mental disorders was 4.27 times more likely to experience presenteeism, 10.17% of compromised overall productivity, and impairment of mental/interpersonal and production demands. Conclusion: The occurrence of common mental disorders was associated with presenteeism, with repercussions in loss of productivity of nursing workers.

RESUMEN Objetivo: Investigar la asociación entre la ocurrencia de trastornos mentales comunes con pérdida de productividad y presentismo en trabajadores de enfermería de un servicio público de salud. Método: Estudio transversal, con 291 trabajadores de la región Centro-Oeste de Brasil. Recolección de datos realizada de octubre de 2019 a enero de 2020, con instrumentos de caracterización sociodemográfica, condiciones laborales y de salud: Self Reporting Questionnaire-20, Standford Presenteeism Scale y Work Limitations Questionnaire. Los datos fueron analizados mediante estadística descriptiva e inferencial, con pruebas de Mann-Whitney y regresión logística, respetando un nivel de significación del 5%. Resultados: La ocurrencia de trastornos mentales comunes fue 4,27 veces más probable de experimentar presentismo, con 10,17% de la productividad general comprometida y daños en las demandas mentales/interpersonales y de producción. Conclusión: La ocurrencia de trastornos mentales comunes se asoció al presentismo, con repercusiones en la pérdida de productividad de los trabajadores de enfermería.

RESUMO Objetivo: Investigar a associação entre a ocorrência de transtornos mentais comuns com a perda de produtividade e o presenteísmo em trabalhadores de enfermagem de um serviço de saúde público. Método: Estudo transversal, com 291 trabalhadores da região Centro-oeste do Brasil. Coleta de dados realizada de outubro de 2019 a janeiro de 2020, com instrumentos para caracterização sociodemográfica, condições laborais e de saúde: Self Reporting Questionnaire-20, Standford Presenteeism Scale e Work Limitations Questionnaire. Os dados foram analisados por estatística descritiva e inferencial, com Testes de Mann-Whitney e regressão logística, respeitando o nível de significância de 5%. Resultados: A ocorrência de transtornos mentais comuns apresentou chances 4,27 maiores para vivenciar o presenteísmo, com 10,17% da produtividade geral comprometida e prejuízos para as demandas mental/interpessoal e de produção. Conclusão: A ocorrência de transtornos mentais comuns esteve associada ao presenteísmo, com repercussões em perda de produtividade dos trabalhadores de enfermagem.

The use of melittin to enhance transgene expression mediated by recombinant adeno-associated virus serotype 2 vectors both in vitro and in vivo / 中西医结合学报

OBJECTIVE@#Melittin, a cell-penetrating peptide, improves the efficiency of many non-viral gene delivery vectors, yet its application in viral vectors has not been well studied. The non-pathogenic recombinant adeno-associated virus (rAAV) vector is an ideal in vivo gene delivery vector. However, its full potential will only be achieved after improvement of its transduction efficiency. To improve the transduction efficiency of rAAV2 vectors, we attempted to develop a melittin-based rAAV2 vector delivery strategy.@*METHODS@#The melittin peptide was inserted into the rAAV2 capsid either in the loop VIII of all viral proteins (VPs) or at the N terminus of VP2. Various rAAV2-gfp or -fluc vectors were subjected to quantitative real-time polymerase chain reaction and Western blot assays to determine their titers and integrity of capsid proteins, respectively. Alternatively, the vectors based on wild-type capsid were pre-incubated with melittin, followed by transduction of cultured cells or tail vein administration of the mixture to C57BL/6 and BALB/c nude mice. In vivo bioluminescence imaging was performed to evaluate the transgene expression.@*RESULTS@#rAAV2 vectors with melittin peptide inserted in the loop VIII of VPs had low transduction efficiency, probably due to dramatically reduced ability to bind to the target cells. Fusing the melittin peptide at the N-terminus of VP2 produced vectors without the VP2 subunit. Interestingly, among the commonly used rAAV vectors, pre-incubation of rAAV2 and rAAV6 vectors with melittin significantly enhanced their transduction efficiency in HEK293 and Huh7 cells in vitro. Melittin also had the ability to increase the rAAV2-mediated transgene expression in mouse liver in vivo. Mechanistically, melittin did not change the vector-receptor interaction. Moreover, cell counting kit-8 assays of cultured cells and serum transaminase levels indicated melittin had little cytotoxicity.@*CONCLUSION@#Pre-incubation with melittin, but not insertion of melittin into the rAAV2 capsid, significantly enhanced rAAV2-mediated transgene expression. Although further in vivo evaluations are required, this research not only expands the pharmacological potential of melittin, but also provides a new strategy to improve gene therapy mediated by rAAV vectors.

In vitro research of oral microscope-assisted implant surface decontamination / 华西口腔医学杂志

OBJECTIVES@#To investigate the effect of oral microscope-assisted surface decontamination on implants in vitro.@*METHODS@#Twelve implants that fell off because of severe peri-implantitis were collected, and decontamination was carried out on the surfaces of implants through curetting, ultrasound, titanium brushing, and sandblasting at 1×, 8×, or 12.8× magnifications. The number and sizes of residues on the implants' surfaces after decontamination were determined, and the decontamination effect was analyzed according to the thread spacing in the different parts of the thread.@*RESULTS@#1) The 8× and 12.8× groups scored lower for implant surface residues than the 1× group (P<0.000 1), and the 12.8× group scored lower than the 8× group (P<0.001); 2) no difference in residue score was found between the wide and narrow thread pitch (P>0.05), and the 8× and 12.8× groups had lower scores than the 1× group (P<0.001); 3) the lowest number of contaminants was observed at the tip of the thread, whereas the highest was observed below the thread, and the difference was significant (P<0.001). However, the thread pitch had no effect on the number of contaminants in different areas (P>0.05); 4) the residue scores of the 8× and 12.8× groups were lower than those of the 1× group at the thread tip and above, sag, and below the thread of the implants (P<0.05).@*CONCLUSIONS@#Residues on the surfaces of contaminated implants can be effectively removed by using an oral microscope. After decontamination, the residues of pollutants were mainly concentrated below the thread of the implants, and the thread pitch of the implants had no significant effect on the residues.

Effect of Medicinal and Edible Herbs in Colorectal Cancer Patients During Chemotherapy / 中国实验方剂学杂志

Malignant tumors are a major health problem in current society. With the improvement of people's living standards and the changes in diet structure， the development trend of gastrointestinal tumors in China is gradually similar to that in developed countries. The incidence and mortality of colorectal cancer （CRC） remain high in China. Although targeted therapy and immunotherapy have greatly improved the prognosis of patients in recent years， chemotherapy is still the main means in clinical practice. However， the adverse reactions of chemotherapy often seriously affect the quality of life of patients， and even lead to treatment interruption， thereby affecting the efficacy. Oral Chinese medicine shows unique advantages in enhancing efficiency and reducing toxicity in CRC patients during chemotherapy， but its poor drug experience not only makes it difficult for patients to take it consistently but also affects the popularization of Chinese medicine at this stage. Medicinal and edible herbs （MEHs） are an important part of Chinese medicine and they are mild， delicious， convenient， affordable， nutritious， and safe. Therefore， they may be more suitable for patients with CRC chemotherapy to adhere to treatment. However， their efficacy is often criticized by clinical practitioners. They are only used in the food and health products industry， and their role as Chinese medicines has not been fully utilized. This paper summarized the common traditional Chinese medicine （TCM） syndromes and treatment methods during CRC chemotherapy， sorted out the nature， flavor， meridian tropism， and efficacy of MEHs， and reviewed the modern pharmacological research results of MEHs by the method of literature statistics. This study finds that the nature， flavor， meridian tropism， and efficacy of MEHs are in good agreement with the common TCM syndromes and treatment methods during CRC chemotherapy. Moreover， many MEHs have the effects of resisting CRC and alleviating the adverse reactions of chemotherapy. Furthermore， the effectiveness and superior efficacy of MEHs in CRC chemotherapy are initially demonstrated from the theoretical level， but high-quality clinical evidence is still needed to support it. The present study discussed the efficiency-enhancing and toxicity-reducing effects and application advantages of MEHs in CRC patients during chemotherapy to provide references for the clinical promotion of MEHs.

The influence of different CT scanning protocols on AI detection efficiency and radiation dose of solid nodules in chest phantom / 中华放射医学与防护杂志

Objective:To investigate the radiation dose and detection efficiency of artificial intelligence (AI) system for solid nodules in chest phantom with different scanning protocols.Methods:A total of 60 simulated nodules with different CT values and diameters were uniformly placed in each lung lobe and lung segment of the anthropomorphic chest phantom. GE Revolution evo CT was used to scan the chest phantom. 64 groups of images with different scanning parameters were collected at the tube voltage of 80, 100, 120, 140 kV, different noise indexes (NI 10-40 with interval 2), and other fixed parameters. The detection result of simulated nodules were recorded on AI software, and the detection rate and false detection rate were calculated, respectively, for different shapes of nodules. The mean volume CT dose index (CTDI vol) and dose length product (DLP) of each scan were recorded. Results:There were no statistically significant differences in the detection rate and false detection rate of spherical nodules and irregular nodules at different tube voltages( P > 0.05), but there were and statistically significant with different noise indices ( F=10.57, 17.77, 9.33, P < 0.001). Different tube voltages had no statistical significance for CTDI vol and DLP ( P > 0.05), while different noise indices had statistical significance for CTDI vol and DLP ( F=59.87, 60.92, P < 0.001). The detection rates of nodules were moderately or weakly correlated with noise indices, CTDI vol and DLP ( r=0.43, 0.56, -0.58, -0.78, P<0.05), but no correlation with tube voltage ( P>0.05). Conclusions:Scanning protocol has an impact on AI detection efficiency of pulmonary nodules. Reasonable scanning parameters should be selected according to different image quality requirements in clinical practice.

Clinical value of ultrasound-guided fine-needle aspiration biopsy in the diagnosis of thyroid cancer and assessment of cervical lymph node metastasis / 中国基层医药

Objective:To explore the clinical value of ultrasound-guided fine-needle aspiration biopsy in the diagnosis of thyroid cancer and assessment of cervical lymph node metastasis.Methods:The clinical data of 90 patients with thyroid cancer who received treatment in Zhoushan Hospital from October 2018 to April 2021 were retrospectively analyzed. All patients underwent a two-dimensional ultrasound examination and ultrasound-guided fine-needle aspiration biopsy before surgery. Taking surgical and pathological diagnosis as the gold standard, the efficiency of two-dimensional ultrasound examination versus ultrasound-guided fine-needle aspiration biopsy in the diagnosis of thyroid cancer and cervical lymph node metastasis and in the identification of benign and maligant lymph nodes were investigated. Multivariate Logistic regression analysis was performed to analyze the correlation between different ultrasound signs and the detection rate of lymph nodes. Results:Pathological results showed that among the 90 patients, 73 patients had thyroid cancer, and 17 patients had benign lesions. Ultrasound-guided fine-needle aspiration biopsy results showed that 70 patients had thyroid cancer, and 20 patients had benign lesions, including 4 cases of missed diagnosis and 2 cases of misdiagnosis. The diagnostic sensitivity, specificity, accuracy rate, and Kappa value were 94.52%, 88.24%, 93.33%, and 0.79, respectively. These were highly consistent with the surgical and pathological diagnosis (Kappa value > 0.75). Two-dimensional ultrasound revealed 69 patients with thyroid cancer and 21 patients with benign lesions, including 7 cases of missed diagnosis and 4 cases of misdiagnosis. The diagnostic sensitivity, specificity, accuracy rate, and Kappa value were 90.41%, 76.47%, 87.78%, and 0.63, respectively. Pathological results revealed that cervical lymph node metastasis occurred in 12 patients, and it did not occur in 78 patients. The diagnostic sensitivity, specificity, accuracy rate, and Kappa value of ultrasound-guided fine-needle aspiration biopsy were 83.33%, 97.50%, 95.65%, and 0.81 respectively. These were highly consistent with surgical and pathological results (Kappa value > 0.75). The diagnostic sensitivity, specificity, accuracy rate, and Kappa value of two-dimensional ultrasound examination were 75.00%, 94.87%, 92.22%, and 0.67, respectively. A total of 156 lymph nodes were detected by ultrasound-guided fine-needle aspiration biopsy, including 103 benign lymph nodes and 53 malignant lymph nodes, with a diagnostic accuracy rate of 94.17% and 96.22%, respectively. A total of 173 lymph nodes were detected by two-dimensional ultrasound, including 111 benign lymph nodes and 62 malignant lymph nodes, with a diagnostic accuracy rate of 91.89% and 91.93%, respectively. There were no significant differences in the diagnostic accuracy of benign and malignant lymph nodes between the two examination methods ( χ2 = 0.42, 0.92, both P > 0.05). Multivariate logistic regression analysis showed that hyperechoic masses, cystic lesions, and internal calcification were significantly correlated with the detection rate of lymph nodes diagnosed by two-dimensional ultrasound and ultrasound-guided fine-needle aspiration biopsy ( OR = 6.64, 5.32, 4.12, 7.07, 5.60, 5.06, all P < 0.05). Conclusion:Ultrasound-guided fine-needle aspiration biopsy has high diagnostic efficiency for thyroid cancer and cervical lymph node metastasis. Ultrasound signs of hyperechoic mass, cystic lesions, and internal calcification are significantly correlated with the detection rate of lymph nodes.

On the construction of private class learning community based on intelligent teaching platform and analysis of its application effects / 中华医学教育探索杂志

In order to improve the effectiveness of private class inquiry with the development of information teaching, the smart teaching platform has been established, with instructional management, curriculum setting, teacher preparation, classroom application, supervision and monitoring modules. Taking the platform as the medium, the small class inquiry learning community of entity curriculum is constructed between students and the teachers. In the eight-year medical teaching, the content of learning cycle is designed according to the entity curriculum, which is issued on cloud platform before class, in class and after class. Students learn basic concepts by themselves in the learning community, explore the application of knowledge under the guidance of teachers, and expand knowledge in class or after class. After having test in teaching procedure, the small class learning community based on smart teaching cloud platform has a submission rate, interaction rate and score rate of more than 90%. It can not only make full use of the advantages of information-based teaching resources, but also build face-to-face learning community in the course teaching, reflecting the emotional interaction of personalized teaching. It's suggested that new approaches to teaching should be student-centered and activity-based, engaging students actively in the learning process, which can promote students' autonomous learning ability and innovative thinking ability.

Analysis of the efficiency and influencing factors of peripheral blood hematopoietic stem cell collection / 中国医师进修杂志

Objective:To investigate the collection efficiency of peripheral blood hematopoietic stem cells and explore its influencing factors.Method:The parameters of the collection process, blood routine indexes and the number of MNC and CD 34+ cells of the product were detected by Fresenius blood cell separator, Mindray blood cell analyzer and BD flow cytometer. A retrospective analysis was performed on 72 patients who underwent autologous peripheral blood hematopoietic stem cell transplantation in Southwest Hospital of Army Medical University from January 2013 to January 2021, including the correlation among gender, age, blood routine indexes, collection circulation volume and MNC and CD 34+ cell count in these cases, and influence of various factors on collection efficiency of peripheral blood stem cells. The correlation among gender, age, blood routine indexes, collection circulation volume and MNC and CD 34+ cell count in 72 cases of autologous transplantation patients, and influence of various factors on collection efficiency of peripheral blood stem cells were analyzed retrospectively. Results:There were no significant differences in collecting efficiency of peripheral blood stem cells among patients with different age, sex and disease type ( P>0.05). The collected MNC count of all patients was positively correlated with the collection cycle count ( r = 0.33, P<0.001) and WBC count after mobilization ( r = 0.41, P<0.001). The number of CD 34+ cells collected was positively correlated with MNC count after mobilization ( r = 0.38, P<0.001) and the amount of white membrane collected ( r = 0.48, P<0.001). Logistic multivariate regression analysis showed that MNC count after mobilization: P<0.001, 95% CI 0.07(0.05 - 0.09), collection cycle amount [ P<0.001, 95% CI 0.00(0.00 - 0.00)] and postharvest total amount [ P<0.001, 95% CI 0.07(0.05 - 0.10)] were the influencing factors of the collected MNC number. Meanwhile, these factorswere also the influencing factors of the collected CD 34+ number (MNC count after mobilization: P<0.001, 95% CI 0.09(0.04 - 0.14); collection cycle amount: P = 0.003, 95% CI 0.00(0.00 - 0.00); postharvest total amount: P = 0.005, 95% CI 0.08(0.03 - 0.14)). Conclusions:The collection efficiency of peripheral blood stem cells varies greatly among individuals. The more MNC counts after mobilization, the more peripheral blood stem cells could be collected. In order to obtain high collection efficiency, it is necessary to adjust the parameters of peripheral blood MNC before collection, and pay attention to the collection circulation quantity, postharvest total amount and white membrane volume.